After 24 h they were analyzed in transwell migration assays as described in the legend to Figure 2, except that the transwell inserts were coated with 2 μg/ml CS-1. Moreover, this pattern correlates with the selective adhesive activity of the ADAM12 disintegrin domain (Figure 1). For scratch wound assays, 24-well dishes were precoated with 10 μg/ml FN, and migration assays were conducted as described in Materials and Methods. Cells were either mock transfected or transfected to express full-length ADAM12, and transwell migration assays (A, C, E, and G) or scratch wound assays (B, D, F, and H) were conducted 24 h after transfection. Cells expressing α4β1 but not α5β1 integrin bind to recombinant ADAM12 disintegrin domain.
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(B) CHO-α4/GFP cells were transfected as indicated and then analyzed in scratch wound assays, as described in the legend to Figure 2, except that 24-well dishes were coated with 2 μg/ml CS-1. Similarly, expression of ADAM12 in CHO-α4/GFP cells caused a reduction in cell motility in both transwell (Figure 2C) and scratch wound (Figure 2D) assays. Expression of ADAM12 reduced the motility of CHOB2-α4 cells in both transwell (Figure 2A) and scratch wound (Figure 2B) assays.
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